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目的利用流式细胞术检测疟原虫感染患者的血液红细胞,建立疟疾诊断的辅助方法。方法采集镜检确诊的疟疾患者血样,同时设正常人血样为阴性对照,通过DRAQ5单染色,利用流式细胞仪进行检测。结果正常人全血、加有DRAQ5荧光染料的正常人全血、未加DRAQ5染料的疟疾患者血样的SSC/FL3-H散点图中未发现有染料标记的红细胞;而在疟原虫感染的患者血样中,有染料DRAQ5标记的细胞被检出。结论流式细胞术可以作为一种新的疟疾辅助诊断技术。
Objective To detect the blood red blood cells of patients infected with Plasmodium by flow cytometry and to establish an auxiliary method for the diagnosis of malaria. Methods Blood samples of patients with malaria diagnosed by microscopic examination were collected. At the same time, normal human blood samples were taken as negative control and detected by flow cytometry by single stain of DRAQ5. Results In normal human whole blood, normal human whole blood supplemented with DRAQ5 fluorescent dye and no dye-labeled erythrocytes were found in the SSC / FL3-H scattergram of blood samples from malaria patients without DRAQ5 dye. In patients with Plasmodium infection In the blood sample, dye labeled DRAQ5 cells were detected. Conclusion Flow cytometry can be used as a new diagnostic technique for malaria.