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目的探讨在胶质瘤的光动力疗法中应用新型光敏剂ATX-S10.Na(Ⅱ)的可能性。方法首先对5种含有不同浓度的ATX-S10.Na(Ⅱ)的鼠和人的胶质瘤细胞中应用波长为670nm的激光照射,应用MTT法测定不同的肿瘤细胞对PDT治疗的反应。接着,在SD大鼠的C6胶质瘤皮下肿瘤模型应用中进行PDT治疗,探讨ATX-S10.Na(Ⅱ)介导的光化学反应所致的抗肿瘤作用。结果体外实验表明,PDT对肿瘤细胞的毒性作用既依靠ATX-S10.Na(Ⅱ)的药物浓度又依靠激光辐照的累计能量。每个细胞系的50%抑制浓度(IC50)波动于5-15μg/ml之间。在皮下肿瘤模型中,PDT治疗导致的肿瘤内部破坏面积随着激光能量的提高而增大,尤其是当照光剂量大于75J/cm时,与对照组相比有统计学意义。结论体外和在体的实验表明,新型光敏剂ATX-S10.Na(Ⅱ)介导的光动力疗法显示出极强的抗胶质瘤作用。
Objective To investigate the possibility of using a new photosensitizer ATX-S10.Na (Ⅱ) in the photodynamic therapy of gliomas. Methods Five kinds of glioma cells containing different concentrations of ATX-S10.Na (Ⅱ) were irradiated with 670nm laser. The response of different tumor cells to PDT was determined by MTT assay. Next, PDT was performed in the SD rat C6 glioma subcutaneous tumor model to investigate the antitumor effect induced by ATX-S10.Na (II) photochemical reaction. Results In vitro experiments showed that the toxic effects of PDT on tumor cells depend not only on the concentration of ATX-S10.Na (Ⅱ) but also on the cumulative energy of laser irradiation. The 50% inhibitory concentration (IC50) of each cell line fluctuated between 5 and 15 μg / ml. In the subcutaneous tumor model, the area of tumor destruction caused by PDT increased with the increase of laser energy, especially when the illumination dose was more than 75 J / cm, which was statistically significant compared with the control group. Conclusion In vitro and in vivo experiments show that the new photosensitizer ATX-S10.Na (Ⅱ) -mediated photodynamic therapy showed a very strong anti-glioma effect.