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合成了N-羟基琥珀酰亚胺-α-萘乙酸酯(SINA)。研究了这一试剂在反相高效液相色谱中作为新的柱前衍生试剂,紫外可见检测,分离测定氨基酸的基本条件。衍生条件简单,方便。RP-HPLC分离了多种氨基酸,检测下限达pmol级,是一优良的柱前衍生试剂
N-hydroxysuccinimide-alpha-naphthaleneacetate (SINA) was synthesized. This reagent was studied as a new pre-column derivatization reagent in reversed-phase high-performance liquid chromatography. The basic conditions for the determination of amino acids were determined by UV-visible detection. Derived conditions are simple and convenient. RP-HPLC separation of a variety of amino acids, detection limit reached pmol level, is an excellent pre-column derivatization reagent