目的:探讨大、小鼠鼻腔毒性病理检查标本制作方法。方法:通过对展片水温度、脱钙时间等关键环节的控制,对大、小鼠鼻腔毒性病理检查进行规范化制片。结果:制作出符合毒性病理学检查的4个切面包括:第一个面上切牙后部面(Posterior part of upper incisors),第二个面切牙乳头面(Incisive papilla),第三个面第二腭嵴面(Second palatine crest),第四个面第一磨牙面(Firstmolar teeth)。结论:运用本制片方法可以很好的制作出组织结构清晰,染色对比鲜明,符合镜检要求的切片。 Objective: To investigate the methods of making nasopharyngeal toxic pathology specimens in rats and mice. Methods: Through the control of key aspects such as water temperature and time of decalcification, the nasopharyngeal toxicity and pathological examination of large and mouse were normalized and made. RESULTS: Four sections were made according to toxicological pathology including the first posterior part of upper incisors, the second incisor papilla, the third face Second palatine crest, Fourth mover firstmolar teeth. Conclusion: This method can be used to produce a good organizational structure clear, stained contrast, in line with the requirements of microscopic examination of the slice.