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目的探讨婴幼儿喘息性疾病患儿外周血单个核细胞(PBMC)表面淋巴细胞功能相关抗原-1(LFA-1)和巨噬细胞分化抗原-1(Mac-1)的表达及相关性,探讨喘息性疾病的免疫学发病机制,鉴别儿童喘息临床表型的炎性指标。方法随机选取在本院住院的3岁以下喘息患儿29例。男17例,女12例。从同期在本院住院的呼吸道感染但不伴有喘息的患儿中随机抽取同年龄患儿26例作为非喘息组,男15例,女11例。从同期在本院儿保门诊体检的健康儿童及外科非感染性疾病患儿中随机抽取同年龄患儿25例作为对照组。男15例,女10例。3组婴幼儿均在治疗前抽取静脉血2 mL,分离PBMC,采用流式细胞术检测PBMC中LFA-1、Mac-1的表达情况。结果喘息组和非喘息组LFA-1的表达[(47.46±13.94)%和(35.88±12.64)%]明显高于对照组[(26.62±10.41)%],喘息组和非喘息组Mac-1的表达[(7.43±3.42)%和(4.53±3.04)%]明显高于对照组[(2.92±1.80)%]。其中喘息组患儿LFA-1和Mac-1的表达高于非喘息组,差异均有统计学意义(Pa<0.01);相关性分析显示LFA-1和Mac-1在喘息组呈正相关(r=0.403,P<0.05)。结论 LFA-1、Mac-1在喘息性疾病发病机制中具有重要作用,检测LFA-1、Mac-1的表达可作为鉴别婴幼儿喘息临床表型的炎性指标。
Objective To investigate the expression and correlation of LFA-1 and Mac-1 on peripheral blood mononuclear cells (PBMCs) in children with asthmatic diseases Immunological pathogenesis of wheezing diseases, inflammatory markers to identify clinical phenotypes of wheezing in children. Methods A total of 29 hospitalized children under 3 years of age with asthma were randomly selected in our hospital. 17 males and 12 females. Twenty-six children of the same age were randomly selected from non-wheezing group, including 15 males and 11 females, from randomly selected children who were hospitalized with respiratory infections but did not have wheezing in our hospital. Twenty-five children of the same age were randomly selected from healthy children and surgical non-infectious diseases in our hospital during the same period as the control group. 15 males and 10 females. Infants and infants in both groups were drawn venous blood 2 mL before treatment, PBMC were isolated, and the expression of LFA-1 and Mac-1 in PBMC was detected by flow cytometry. Results The expression of LFA-1 in asthmatic group and non-asthmatic group [(47.46 ± 13.94)% vs (35.88 ± 12.64)%] was significantly higher than that in control group [(26.62 ± 10.41)%] (7.43 ± 3.42)% and (4.53 ± 3.04)%, respectively, were significantly higher than those in the control group (2.92 ± 1.80%). The expression of LFA-1 and Mac-1 in wheezing group was higher than that in non-wheezing group (P <0.01). Correlation analysis showed that there was a positive correlation between LFA-1 and Mac-1 in wheezing group = 0.403, P <0.05). Conclusion LFA-1 and Mac-1 play an important role in the pathogenesis of wheezing diseases. Detecting the expression of LFA-1 and Mac-1 can be used as an inflammatory index to differentiate the clinical phenotype of asthmatic children.