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目的探讨红细胞膜磷脂组分的高效液相测定方法。方法选用 Sｐｈｅｒｉｓｏｒｂ硅胶色谱柱，流动相为乙腈－甲醇－８５％磷酸（１００：１０：１.８Ｖ／Ｖ／Ｖ），波长２０３ｎｍ。结果４分钟内完全分离膜的四种磷脂组分磷脂酰丝氨酸（ＰＳ）、磷脂酰乙醇胺（ＰＥ）、磷脂酰胆碱（ＰＣ）和神经鞘磷脂（ＳＭ），最低检出限为１μｇ／ｍｌ，至少在４μｇ－２０００μｇ／ｍｌ范围内标准品浓度与峰面积积分值呈线性关系，ＰＳ、ＰＥ、ＰＣ、ＳＭ的回收率分别为９８.１±２.３％、９９．８±０.７％、９９.４±１.８％、９０．６±２．２％。结论本法适于临床检测和科研探索。
Objective To investigate the method of high performance liquid chromatography of erythrocyte membrane phospholipid. Methods Spherisorb silica gel column was used. The mobile phase consisted of acetonitrile-methanol-85% phosphoric acid (100: 10: 1.8 V / V / V) with a wavelength of 203 nm. Results The four phospholipid components, phosphatidylserine (PS), phosphatidylethanolamine (PE), phosphatidylcholine (PC) and sphingomyelin (SM) were completely separated within 4 minutes with the detection limit of 1μg / ml , At least in the range of 4μg-2000μg / ml standard concentration and peak area integral value of a linear relationship, PS, PE, PC, SM recovery rates were 98.1 ± 2.3%, 99.8 ± 0.7 %, 99.4 ± 1.8%, 90.6 ± 2.2%. Conclusion This method is suitable for clinical testing and scientific research.