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已知酸-染料法(Acid-dye technique)用于测定微量的莨菪烷类生物碱时,只要对介质中的pH和所用染料作适当选择,就不需预先分离即可测得各个生物碱。作者曾于pH3时用麝香草酚蓝测定阿托品,托品无干扰。本文研究在不作预先分离的条件下,莨菪碱和东莨菪碱的微量测定中,pH和染料选择的影响。结果表明,在各个不同的pH值下,莨菪碱和东莨菪碱均与溴麝香草酚蓝生成复合物。但在pH5.6时用溴麝香草酚蓝测定莨菪碱的标准曲线,与PH 6.6时用溴甲酚紫测定莨菪碱的标准曲线几乎完全重叠,两曲线斜率分别为2.564和2.578,取其平均值为2.57。为了便于计算,在使用溴麝香草酚
Known acid-dye method (Acid-dye method) for the determination of trace amounts of tropane alkaloids, as long as the pH of the medium and the dye used to make the appropriate choice, without prior separation of each alkaloid can be measured. The authors at the pH3 with thymol blue determination of atropine, care products without interference. This article investigates the effects of pH and dye selection in the minimal determination of scopolamine and scopolamine without prior isolation. The results showed that both scopolamine and scopolamine both formed complexes with bromothymol blue at various pH values. However, at pH 5.6, the standard curve of hyoscyamine was determined with bromothymol blue and almost completely overlap with the standard curve of hyoscyamine at pH 6.6, with the slope of the two curves being 2.564 and 2.578, respectively, The value is 2.57. For ease of calculation, bromothymol is used