艰难梭菌的血清学分型通常用玻片凝集或PAGE法。玻片凝集分型分出10个血清型,而PAGE分型除A型分出12个不同亚型(A_1～A_(12))外,其余9型与玻片法有极好的相关性。本文报告采用ELISA对临床粪便标本中分离的艰难梭菌直接作血清型别分析。从收集的菌株中选取141株用ELISA作方法学考评,其中包括10株参照菌株、12株A亚型及120株其他血清型菌株。所用抗血清分别为抗A_1、A_5、A_8、A_9、A_(10)、C、D、F、H、G及K型共11种。将细菌以pH9.6碳酸 Serological typing of C. difficile is commonly done by slide agglutination or PAGE. There were 10 serotypes in the slide agglutination group, but there were 12 isolates in the PAGE genotype (A_1-A_ (12)), and the other 9 types had excellent correlation with the slide method. This article reports the use of ELISA for direct analysis of serotypes of Clostridium difficile isolated from clinical stool specimens. 141 strains from the collected strains were screened by ELISA method, including 10 reference strains, 12 strains of A subtype and 120 strains of other serotypes. The antisera used were 11 anti-A_1, A_5, A_8, A_9, A_ (10), C, D, F, H, G and K types respectively. Bacteria were pH 9.6 carbonated