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利用特三矮2号/CB组合构建了重组自交系群体(RI)。以60个RFLP标记检测142个纯系的基因型。在含有NaCl的电导率为12dS/m的培养液中鉴定这些纯系的耐盐性。结果表明,RI群体的耐盐性出现超亲分离。构建了一张覆盖11条染色体、含52个标记位点的连锁图。仅检测到一个位于第5染色体的位点(RG13)显著与耐盐性有连锁。该位点的表型贡献率为11.6%。来自母本的该位点可提高耐盐性。分别对RG13与其它59个标记位点间的互作做检测,仅发现3对互作显著,即RG13×RG104;RG13×RG143;RG13×RG716。当来自母本的RG13分别与来自父本的RG104和RG143重组时,均明显提高耐盐性。分别来自母本的RG13和RG716能产生提高耐盐性的互作。这些基因互作结果为超亲分离提供了理论依据。
Recombinant inbred lines (RIs) were constructed by using a combination of special three dwarf 2 / CB. The genotypes of 142 pure lines were tested with 60 RFLP markers. Salt tolerance of these pure lines was identified in broth containing NaCl at a conductivity of 12 dS / m. The results showed that the salt tolerance of RI population appeared super-parents separation. Constructed a chain map covering 11 chromosomes with 52 marker sites. Only one locus on chromosome 5 (RG13) was detected to be significantly linked to salt tolerance. The phenotype contribution rate of this site was 11.6%. This site from the female parent can improve salt tolerance. The interaction between RG13 and other 59 marker sites were detected respectively, and only three pairs of interactions were found, namely RG13 × RG104; RG13 × RG143; RG13 × RG716. When RG13 from the female parent was recombined with the RG104 and RG143 from the male parent, the salt tolerance was significantly improved. RG13 and RG716 from the female parent, respectively, can produce an interaction that enhances salt tolerance. The results of these gene interactions provide the theoretical basis for the super-separation.