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目的:研究小檗碱对K562细胞是否具有抑制增殖、促进分化、诱导凋亡的作用,为临床应用黄连治疗慢性粒细胞白血病提供理论依据。方法:MTT比色法、克隆形成实验检测小檗碱对K562细胞增殖的影响;瑞氏-吉姆萨染色观察细胞凋亡的形态改变;DNA琼脂糖凝胶电泳检测晚期细胞凋亡;流式细胞术分析细胞周期分布,细胞表面分化抗原表型检测分析细胞分化。结果:小檗碱对K562细胞的生长有抑制作用;小檗碱与Ara-C联用存在一定的协同效应;小檗碱处理K562细胞48h后能明显促进其向红系、粒系、巨核系分化,随着作用时间的延长小檗碱诱导K562细胞发生凋亡的百分比逐渐增加。结论:小檗碱能有效地抑制K562细胞的增殖,其作用可能是通过诱导K562细胞发生G0/G1期和(或)G2期阻滞并进一步诱导其凋亡和分化实现的。
OBJECTIVE: To study whether Berberine can inhibit proliferation, promote differentiation and induce apoptosis in K562 cells and provide a theoretical basis for the clinical application of Coptis chinensis in the treatment of chronic myeloid leukemia. Methods: MTT assay and colony formation assay were used to detect the effects of berberine on the proliferation of K562 cells. Wright-Giemsa staining was used to observe the morphological changes of apoptosis cells. DNA agarose gel electrophoresis was used to detect the apoptosis of late-stage cells. Flow cytometry Analysis of cell cycle distribution, cell surface differentiation antigen phenotype detection of cell differentiation. Results: Berberine inhibited the growth of K562 cells. There was a synergistic effect between berberine and Ara-C. Berberine treated K562 cells for 48 h, Differentiation, with the extension of the role of berberine-induced apoptosis in K562 cells gradually increased the percentage. CONCLUSION: Berberine can effectively inhibit the proliferation of K562 cells, and its effect may be through the induction of G0 / G1 and / or G2 arrest and further induction of apoptosis and differentiation in K562 cells.