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以玉米自交系郑58(抗旱性强)和丹340(抗旱性弱)为材料,采用盆栽控水试验,设置中度干旱和正常灌水处理,研究干旱胁迫对玉米自交系苗期、抽雄吐丝期和灌浆期叶片叶绿素荧光指标的影响。结果表明,不同生育时期,玉米自交系叶片光系统Ⅱ(PSⅡ)的最大荧光(Fm)、原初最大光能利用效率(Fv/Fm)、潜在光化学效率(Fv/F0)、有效光化学量子产量(Fv’/Fm’)、实际光化学量子效率(ΦPSⅡ)和光化学猝灭系数(q P)均随干旱胁迫的加剧而下降;初始荧光(F0)和非光化学猝灭系数(q N)均随干旱胁迫的加剧而升高。与丹340相比,郑58的Fm、Fv/Fm、Fv/F0、Fv’/Fm’降幅均相对较小。不同时期干旱胁迫下,郑58的Fm、Fv/Fm、Fv/F0、Fv’/Fm’、ΦPSⅡ和q P均高于丹340。与丹340相比,干旱胁迫下,郑58光系统Ⅱ具有较高的光能转化和利用效率、实际光化学量子效率和有效光化学量子产量,通过调节碳同化能力和激发能利用效率,有效地耗散过剩光能减轻光抑制,保护光合机构,维持较高的光合性能。光系统Ⅱ中Fm、Fv/Fm和Fv/F0可以用来鉴定玉米自交系抗旱性。
Maize inbred lines Zheng 58 (strong drought resistance) and Dan 340 (drought resistance weak) as materials, using potted water control experiment, setting moderate drought and normal irrigation treatment of drought stress on maize inbred lines seedling, tasseling Effect of Chlorophyll Fluorescence Index at Leaf Silking and Grain Filling Stage. The results showed that the maximum fluorescence (Fm), initial maximum photosynthetic light utilization efficiency (Fv / Fm), potential photochemical efficiency (Fv / F0), effective photochemical quantum yield of maize inbred lines at different growth stages, (Fv ’/ Fm’). The actual photochemical quantum efficiency (ΦPSⅡ) and photochemical quenching coefficient (q P) decreased with the increase of drought stress. The initial fluorescence (F0) and non-photochemical quenching coefficient Drought stress intensified and increased. Compared with Dan 340, the decrease of Fm, Fv / Fm, Fv / F0, Fv ’/ Fm’ of Zheng 58 was relatively small. The Fm, Fv / Fm, Fv / F0, Fv ’/ Fm’, ΦPSⅡ and q P of Zheng 58 were higher than those of Dan 340 under drought stress at different periods. Compared with Dan 340, under drought stress, Zheng 58 photosystem II had high light energy conversion and utilization efficiency, actual photochemical quantum yield and effective photochemical quantum yield. By adjusting carbon assimilation capacity and energy utilization efficiency, Scattered surplus light can reduce light suppression, protect photosynthetic institutions, to maintain high photosynthetic properties. Photosystem Ⅱ Fm, Fv / Fm and Fv / F0 can be used to identify the drought resistance of maize inbred lines.