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目的:探讨精液优化处理后孵育不同时间受精对体外受精-胚胎移植(in vitro fertilization-embryo transfer,IVF-ET)治疗的实验室指标及临床结局的影响。方法:选取2011年7月~2012年7月因女方输卵管因素导致不孕并行IVFET治疗的患者160例为研究对象。按受精时男方精液优化后孵育时间不同(1~2 h、2~3 h、3~4 h、4~6 h)而随机分为4组,每组40例。比较4组的MⅡ卵受精率、正常受精率、正常卵裂率、优质胚胎率、可用胚胎率、临床妊娠率、着床率、流产率。结果:分别比较4组患者的MⅡ卵受精率、正常受精率、正常卵裂率、优质胚胎率、可用胚胎率、临床妊娠率、着床率及流产率,差异均无统计学意义(P>0.05)。结论:IVF-ET中精液优化后孵育6 h内受精并不影响实验室指标及临床结局。
OBJECTIVE: To investigate the effects of different fertilization after semen optimization treatment on laboratory parameters and clinical outcomes in vitro fertilization-embryo transfer (IVF-ET). Methods: From July 2011 to July 2012, 160 women with IVFET-treated infertility due to female tubal factor were selected as the study objects. According to different fertilization time (1-2 h, 2-3 h, 3-4 h, 4-6 h), the male semen was divided into 4 groups randomly, 40 in each group. The fertilization rate, normal fertilization rate, normal cleavage rate, high quality embryo rate, available embryo rate, clinical pregnancy rate, implantation rate and miscarriage rate were compared among the four groups. Results: There was no significant difference in M Ⅱ egg fertilization rate, normal fertilization rate, normal cleavage rate, high quality embryo rate, available embryo rate, clinical pregnancy rate, implantation rate and abortion rate among the four groups (P> 0.05). CONCLUSIONS: Fertilization within 6 h of sperm optimization in IVF-ET does not affect laboratory parameters and clinical outcome.