组织化学研究,死后取材的时间是很重要的,死后时间过长,组织产生自溶,对组织中酶的观察产生一定影响。当然组织愈新鲜愈好,但限于条件一般要求取材不超过死后6小时。心肌组织在正常情况下溶酶体数量不多,自溶现象产生得比较慢,但在死后6小时取材对心肌组织化学酶的观察是否有影响则不清楚。为此我们设计了本实验,以观察大白鼠在死后2—12小时内心肌琥珀酸脱氢酶、酸性磷酸酶的变化。实验方法: 采用成年大白鼠20只,用拉脊椎方法处死,不放血,摹拟人死后情况,放置于室温18℃左右,除死后6小时杀死8只外,余在2小时、4小时、12小时各杀4只。每次处 Histochemistry, the time of death after drawing is very important, too long after the death, the organization produces autolysis, the organization of enzymes have some impact on the observation. Of course, the more fresh tissue better, but limited to the conditions generally require no more than 6 hours after death. Myocardial tissue under normal circumstances, a small number of lysosomes, autolysis phenomenon is relatively slow, but after 6 hours of death on the myocardial tissue chemical enzyme whether the impact is not clear. To this end, we designed this experiment to observe the rat myocardial succinate dehydrogenase, acid phosphatase changes within 2-12 hours after death. Experimental methods: Twenty adult rats were sacrificed by pulling the spine, without bleeding. After the death was simulated, the animals were placed at room temperature of about 18 ℃. Eight animals were killed at 6 hours after the death, while the animals were killed at 2 hours and 4 hours , Kill 12 in 12 hours each. Every time