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目的探讨外源性NO供体亚硝基铁氰化钠(SNP)对旋毛虫病小鼠肝脏、外周血中抗氧化酶活性及脂质氧化的影响。方法采用消化法分离旋毛虫肌幼虫,感染BALB/c小鼠,400条/只。42 d后,收集感染鼠及正常小鼠外周血、肝脏。设A组(感染鼠肝脏匀浆物+SNP)、B组(正常鼠肝脏匀浆物+SNP)、C组(感染鼠外周血+SNP)、D组(正常鼠外周血+SNP)。每组内设5个SNP反应终浓度,分别为0(空白对照)、2、5、10、30μmol/L,37℃反应30 min,测定各组超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)及脂质氧化(MDA)含量。结果与正常鼠相比,旋毛虫感染鼠肝脏、外周血中SOD、CAT、GSH-Px活性上升,MDA含量增加,差异均有统计学意义(P均<0.05);在A、B组中,10、30μmol/L SNP作用下SOD、CAT、GSH-Px活性较空白对照降低(P均<0.05);与2~30μmol/L SNP反应后,MDA含量较空白对照明显升高(P均<0.05)。在C、D组中,30μmol/LSNP作用下SOD、CAT、GSH-Px活性显著下降,MDA浓度升高,与空白对照比较差异均有统计学意义(P均<0.01)。在2~30μmol/L浓度区间,SNP浓度与旋毛虫感染鼠肝脏、外周血中SOD、CAT、GSH-Px活性呈负相关(r肝SOD=-0.901,r肝CAT=-0.802,r肝GSH-Px=-0.847,r血SOD=-0.899,r血CAT=-0.685,r血GSH-Px=-0.635,P均<0.05),与MDA含量呈正相关(r肝MDA=0.697,r血MDA=0.764,P均<0.05)。结论旋毛虫感染可致小鼠肝脏、外周血中活性氧自由基产生增加,SOD、CAT、GSH-Px活性升高;外源性NO可降低旋毛虫感染小鼠SOD、CAT、GSH-Px活性,抑制小鼠的抗氧化能力。
Objective To investigate the effects of exogenous NO donor nitrosylated sodium cyanide (SNP) on antioxidant enzyme activity and lipid oxidation in liver and peripheral blood of mice with Trichinella spiralis. Methods The Trichinella spiralis larvae were isolated by digestion and infected with 400 BALB / c mice. After 42 days, peripheral blood and liver of infected mice and normal mice were collected. Group A (infected mouse liver homogenate + SNP), group B (normal mouse liver homogenate + SNP), group C (infected rat peripheral blood + SNP) and group D (normal mouse peripheral blood + SNP). Five SNP final concentrations in each group were set as 0 (blank control), 2, 5, 10, and 30μmol / L, respectively. The reaction was carried out at 37 ℃ for 30 min. The superoxide dismutase (SOD) (CAT), glutathione peroxidase (GSH-Px) and lipid oxidation (MDA) content. Results Compared with normal mice, the activities of SOD, CAT and GSH-Px in the liver and peripheral blood of Trichinella spiralis infected mice increased and the content of MDA increased (all P <0.05). In group A and B, The activities of SOD, CAT and GSH-Px in 10 and 30μmol / L SNP groups were lower than those in blank control group (all P <0.05). After reacting with 2 ~ 30μmol / L SNP, ). In the groups C and D, the activities of SOD, CAT, GSH-Px and the concentrations of MDA were significantly decreased under the action of 30μmol / L LSNP compared with the control group (all P <0.01). In the range of 2 ~ 30μmol / L, the concentration of SNP was negatively correlated with the activity of SOD, CAT and GSH-Px in the liver and peripheral blood of Trichinella spiralis infected mice (r liver SOD = -0.901, r liver CAT -Px = -0.847, r blood SOD = -0.899, r blood CAT = -0.685, r GSH-Px = -0.635, all P <0.05), and positively correlated with MDA content = 0.764, P <0.05). Conclusions Trichinella spiralis infection can increase the production of reactive oxygen species (ROS) and the activities of SOD, CAT and GSH-Px in the liver of mice and exogenous NO can reduce the activities of SOD, CAT and GSH-Px in mice infected with Trichinella , Inhibit the mouse’s antioxidant capacity.