论文部分内容阅读
目的探讨二氧化氯对肠道腺病毒41型(AdV41)核酸的破坏在灭活病毒中的作用,并对病毒基因组受损区域进行定位,阐明二氧化氯灭活腺病毒的消毒规律和机制。方法在25℃,pH 7.2条件下,以不同剂量(0.1、0.5、0.7、1.0、2.0 mg/L)的二氧化氯对AdV41作用不同时间(0、0.5、1、2、3、4、5、7、10、20、30、60 min);采用大片段逐步步移PCR(long-overlapping PCR)分析二氧化氯对病毒全基因组的损伤,以细胞培养法检测病毒感染性。结果二氧化氯浓度变化符合幂函数Ct=a0×e-kt规律;病毒的灭活率随着Ct值的增加而逐渐增高;当Ct=0.773 mg(/L.min)时,二氧化氯对AdV41可以达到4log的灭活对数值(即灭活率为99.99%)。AdV41基因组各部分对二氧化氯的抵抗力不同,其5′的1～2 081 nt区域的破坏与AdV41感染性的消失一致。结论 AdV41基因组5′的1～2 081 nt区域对二氧化氯最为敏感,且与病毒的感染性相关;在25℃,pH 7.2时二氧化氯灭活4log AdV41的Ct值为0.773 mg(/L.min)。
Objective To investigate the role of chlorine dioxide on the inactivation of adenovirus type 4 virus (AdV41) in inactivated virus and to locate the damaged regions of the virus genome and elucidate the disinfection mechanism and mechanism of chlorine dioxide inactivated adenovirus. Methods Different concentrations of chlorine dioxide (0.1,0.5,0.7,1.0,2.0 mg / L) were applied to AdV41 at different temperatures (0, 0.5, 1, 2, 3, 4, , 7, 10, 20, 30, 60 min). Whole-genome DNA damage caused by chlorine dioxide was analyzed by long-overlap PCR. The virus infectivity was detected by cell culture. Results The change of concentration of chlorine dioxide was in accordance with the law of power Ct = a0 × e-kt. The inactivation rate of virus increased gradually with the increase of Ct value. When Ct = 0.773 mg (/L.min) AdV41 can reach a 4 log inactivation logarithm (ie an inactivation rate of 99.99%). The resistance of different parts of AdV41 genome to chlorine dioxide was different. The damage of 1 081 nt of 5 ’region was consistent with the disappearance of AdV41 infectivity. Conclusion The region of 1 081 nt in the 5 ’region of AdV41 genome is the most sensitive to chlorine dioxide and is associated with the infectivity of the virus. The Ct value of inactivated 4 log AdV41 of chlorine dioxide at pH 7.2 at 25 ℃ is 0.773 mg / .min).