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为了检测鲤春季病毒血症病毒(SVCV)DNA,用RT—PCR扩增技术和在质粒和噬菌体载体中克隆的cDNA构建了探针。选择并合成了可以扩增SVCV M和G基因的特异性引物。用非放射性探针和RT-PCR研究了检测来源于鱼感染细胞培养物和病原材料中病毒RNA的敏感性和特异性。用参考株Fijan作为补充,在RT—PCR试验中用2个SVCV株测定了用引物扩增的效力。在相同条件下,从M2株扩增的
To detect the Cyprinus carpiovirus (SVCV) DNA, probes were constructed using RT-PCR amplification techniques and cDNA cloned in plasmid and phage vectors. The specific primers that can amplify SVCV M and G genes were selected and synthesized. The non-radioactive probe and RT-PCR were used to examine the sensitivity and specificity of detecting viral RNAs derived from fish-infected cell cultures and pathogenic materials. Supplemented with the reference strain Fijan, two SVCV strains were used in the RT-PCR assay to measure the efficiency of amplification with primers. Under the same conditions, M2 strain was amplified