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为评价人参粗提取物及单体的药效活性,按比浊法进行血小板聚集测定,结果报告如下。1材料与方法1.1试剂:Tyrode buffered的配置:137mmol/L NaCl;0.3mmol/L Na2HPO4;2mmol/L KCl;12mmol/L NaHCO3;0.35%牛血清;1mmol/L CaCl2;5mmol/L Hepes。诱导剂:胶原:鼠尾胶原(Sigma,1mg/mL);麻醉剂:戊巴比妥钠(1%),30～40mg/kg。抗凝剂:枸橼酸钠(3.8%),血液和抗凝剂比例(9:1)。
To evaluate the ginseng crude extract and monomer pharmacodynamic activity, according to turbidimetric platelet aggregation assay, the results reported below. 1 Materials and methods 1.1 Reagents: Tyrode buffered configuration: 137mmol / L NaCl; 0.3mmol / L Na2HPO4; 2mmol / L KCl; 12mmol / L NaHCO3; 0.35% bovine serum; Inducers: collagen: rat tail collagen (Sigma, 1 mg / mL); anesthetic: sodium pentobarbital (1%), 30-40 mg / kg. Anticoagulants: Sodium citrate (3.8%), blood to anticoagulant ratio (9: 1).