白及种子萌发及原球茎发育过程的细胞组织学观察

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为探讨白及种子发育成苗的机制,对组织培养条件下种子萌发过程和原球茎发育过程进行了形态学和石蜡切片细胞组织学观察。结果表明:白及种子吸水膨胀后,出现极性,远离胚柄端分化出绿色芽点,芽点发育成子叶,同时种胚突破种皮形成原球茎,另一端则出现成群的白色绒毛状假根。原球茎顶端分生组织的发育遵循原套-原体学说,内部分化出多束维管束后,白及由原球茎过渡到根状茎阶段。其中根原基的发育始于根状茎维管束,为内起源。当根状茎逐渐膨大,内部基本分生组织分化为成熟薄壁组织后,假鳞茎开始形成。白及种子的发育共经过种胚、原球茎、根状茎和假鳞茎4个阶段。 In order to explore the mechanism of seedling development in white and seed, morphological and paraffin section histological observations of seed germination and protocorm development under tissue culture conditions were carried out. The results showed that the white and seeds became polar when they were swollen by water and differentiated into green shoots away from the end of the embryo and the cotyledons developed into cotyledons. At the same time, the embryo broke the seed coat to form the protocorm, while the other end appeared white flocks rhizoid. The development of protocorms at the apical meristem conforms to the principle of protocorm-protoplasm. After internal differentiation of multiple vascular bundles, the transition from rhizomes to rhizomes is completed. The development of root primordium began in the rhizome vascular bundle, for the internal origin. When the rhizome gradual enlargement, the internal basic meristem differentiation into mature parenchyma, pseudobulb began to form. White and seed development through a total of four kinds of embryo, protocorm, rhizomes and pseudobulb.
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