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AIM:To investigate the alteration of the annexin I subcellularlocalization in esophageal squamous cell carcinoma(ESCC)and the correlation between the translocation and thetumorigenesis of ESCC.METHODS:The protein localization of annexin Ⅰ wasdetected in both human ESCC tissues and cell line via theindirect immunofluorescence strategy.RESULTS:In the normal esophageal epithelia the annexinI was mainly located on the plasma membrane and formeda consecutive typical trammels net.Annexin I protein alsoexpressed dispersively in cytoplasm and the nuclei withoutspecific localization on the nuclear membrane.In esophagealcancer annexin I decreased very sharply with scattereddisappearance on the cellular membrane,however ittranslocated and highly expressed on the nuclear membrane,which was never found in normal esophageal epithelia.In,cultured esophageal cancer cell line annexin I protein wasalso focused on the nuclear membrane,which was consistentwith the result from esophageal cancer tissues.CONCLUSION:This observation suggests that thetranslocation of annexin I protein in ESCC may correlatewith the tumorigenesis of the esophageal cancer.
AIM: To investigate the alteration of the annexin I subcellular localization in esophageal squamous cell carcinoma (ESCC) and the correlation between the translocation and the tumorigenesis of ESCC. METHODS: The protein localization of annexin I was detected in both human ESCC tissues and cell line via the direct immunofluorescence strategy .RESULTS: In the normal esophageal epithelia the annexin I was mainly located on the plasma membrane and formed a consecutive typical trammels net. Annexin I protein alsoexpressed dispersively in cytoplasm and the nuclei withoutspecific localization on the nuclear membrane. esophagealcancer annexin I decreased very sharply with scattereddisappearance on the cellular membrane, however ittranslocated and highly expressed on the nuclear membrane, which was never found in normal esophageal epithelia., cultured esophageal cancer cell line annexin I protein wasalso focused on the nuclear membrane, which was consistent with the result from esophageal cancer tissues.C ONCLUSION: This observation suggests that the transfer of annexin I protein in ESCC may correlate with the tumorigenesis of the esophageal cancer.