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将乙型脑炎SA14 14 2减毒株 (简称 14 2株 )病毒液和灭活 14 2株病毒液皮下和腹腔免疫小白鼠 ,P3株死苗作对照 ,测免疫效价 ,结果 14 2株病毒液的效力为 1 8× 10 -5～ 6 .1× 10 -6ID50 /ml,灭活 14 2株病毒液为 9 9× 10 -1～ 7 2× 10 -3 ID50 /ml,P3株死苗为 4 0× 10 -3 ～ 2 2× 10 -4 ID50 /ml。同时免后 14天采血用PRNT法测抗体 ,结果为 14 2株病毒液 1∶2 (皮下和腹腔 ) ;灭活 14 2株病毒液 <1∶2 ,P3株死苗为皮下 1∶2、腹腔 1∶10。结果表明 :14 2株在小白鼠体内的免疫原性远远高于灭活 14 2株病毒液和P3株死疫苗 ,14 2株经灭活后对小白鼠基本无保护作用。 14 2株抗体水平不高 ,而免疫力远高于P3株死苗 ,说明 14 2株与P3株死疫苗免疫性质不同 ,有细胞免疫参与。
The subcutaneously and intraperitoneally immunized mice of strain 14 of SA14 14 2 (14 2 strains) and the inactivated mouse of subcutaneously and intraperitoneally 14 strains of virus were used as control to test the immune titer. Results of 14 2 strains The potency of the virus solution was 18 × 10 -5 ~ 6 .1 × 10 -6 ID50 / ml, and inactivated 14 2 strains of virus were 9 9 × 10 -1 ~ 7 2 × 10 -3 ID50 / ml. P3 strain died The seedlings were 4 × 10 -3 to 2 × 10 -4 ID50 / ml. At the same time 14 days after immunization with PRNT method to detect antibodies, the results of 14 2 strains of virus 1: 2 (subcutaneous and abdominal); inactivated 14 2 strains of virus <1: 2, P3 plants dead seedling 1: 2, Intraperitoneal 1:10. The results showed that the immunogenicity of 14 2 strains in mice was much higher than that in inactivated 14 2 strains of virus and strain P3, and 14 2 strains had no protective effect on mice after inactivation. 14 2 antibody levels are not high, but immunity is much higher than P3 dead seedlings, indicating that 14 2 strains and P3 dead vaccine immune properties are different, with cellular immune involvement.