论文部分内容阅读
以单分散交联聚氯甲基苯乙烯为基质，先后与乙二胺和(－葡萄糖酸内酯反应，得到了亲水性能良好的排阻色谱固定相，其表面羟基含量高达5.4mmol/g。本文评价了改性后树脂的亲水性、溶剂匹配性、耐压性能及化学稳定性。用三羟甲基氨基甲烷（Tris）缓冲溶液（pH=7.0）为流动相，蛋白质混合样的分离遵循排阻色谱分离机理。聚苯乙烯和蛋白质标准样的排阻色谱校正曲线线性范围分别为9(102(2(105、1(104(6.6(104，两者截距的差异是由于两者分子形状的不同和固定相在不同流动相中溶胀性能不同所致。
Monodisperse cross-linked polychloromethyl styrene as matrix, with ethylenediamine and (- gluconolactone reaction, get a good hydrophilicity of the exclusion chromatography stationary phase, the surface hydroxyl content of up to 5.4mmol / g In this paper, the hydrophilicity, solvent compatibility, pressure resistance and chemical stability of modified resin were evaluated.Using Tris buffer (pH = 7.0) as mobile phase, The separation followed the exclusion chromatographic separation mechanism.The linearity of the exclusion chromatography calibration curve for polystyrene and protein standards was 9 (102 (2 (105,1 (104 (6.6 (104, 104, the difference between the two intercept is due to two The molecular shape of the different and stationary phase in different mobile phase swelling due to different properties.