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Objective Very early-onset coronary artery disease(CAD) is a great challenge in cardiovascular medicine throughout the world,especially regarding its early diagnosis.This study explored whether circulating micro RNAs(mi RNAs) could be used as potential biomarkers for patients with very early-onset CAD.Methods We performed an initial screening of mi RNA expression using RNA isolated from 20 patients with angiographically documented very early-onset CAD and 20 age-and sex-matched normal controls.For further confirmation,we prospectively examined the mi RNAs selected from 40 patients with very early-onset CAD and 40 angiography-normal controls.Results A total of 22 overexpressed mi RNAs and 22 underexpressed mi RNAs were detected in the initial screening.RT-q PCR analysis of the mi RNAs obtained from the initial screening revealed that four mi RNAs including mi R-196-5p,mi R-3163-3p,mi R-145-3p,and mi R-190a-5p exhibited significantly decreased expression in patients compared with that in controls(P<0.05).The areas under the receiver operating characteristic curve for these mi RNAs were 0.824(95% CI,0.731-0.917;P<0.001),0.758(95% CI,0.651-0.864;P<0.001),0.753(95% CI,0.643-0.863;P<0.001),and 0.782(95% CI,0.680-0.884;P<0.001),respectively,in the validation set.Conclusion To our knowledge,this is an advanced study to report about four serum mi RNAs(mi R-196-5p,mi R-3163-3p,mi R-145-3p,and mi R-190a-5p) that could be used as novel biomarkers for the diagnosis of very early-onset CAD.
Objective Very early-onset coronary artery disease (CAD) is a great challenge in cardiovascular medicine throughout the world, especially for its early diagnosis. This study explored whether circulating microRNAs (miRNAs) could be used as potential biomarkers for patients with very early -onset CAD. Methods We performed an initial screening of mi RNA expression using RNA isolated from 20 patients with angiographically documented very early-onset CAD and 20 age-and sex-matched normal controls. For further confirmation, we prospectively examined the mi RNAs selected from 40 patients with very early-onset CAD and 40 angiography-normal controls. Results A total of 22 overexpressed mi RNAs and 22 underexpressed mi RNAs were detected in the initial screening. RT-q PCR analysis of the mi RNAs obtained from the initial screening revealed that four mi RNAs including mi R-196-5p, mi R-3163-3p, mi R-145-3p, and mi R-190a-5p showed significantly reduced expression in patients compared with that in controls (P <0.05). The areas under the receiver operating characteristic curve for these mi RNAs were 0.824 (95% CI, 0.731-0.917; P <0.001), 0.758 (95% CI, 0.651-0.864; 0.753 (95% CI, 0.643-0.863; P <0.001), and 0.782 (95% CI, 0.680-0.884; P <0.001) respectively, in the validation set. Confidence To our knowledge, this is an advanced study to report about four serum mi RNAs (mi R-196-5p, mi R-3163-3p, mi R-145-3p, and mi R-190a-5p) that could be used as novel biomarkers for the diagnosis of very early-onset CAD.