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以草鱼肾细胞系(CIK)和草鱼呼肠孤病毒为材料,采用噻唑蓝比色法分先给药后感染、先感染后给药、药物与病毒共孵育后感染3组进行抗草鱼呼肠孤病毒药物的筛选,建立了体外抗草鱼呼肠孤病毒药物筛选的细胞模型。先给药后感染试验组的结果表明,穿琥宁在阻断草鱼呼肠孤病毒吸附和侵入细胞时效果最强,其次是利巴韦林和板蓝根,黄芩的阻断效果最弱;而在先感染后给药试验组中,穿琥宁、利巴韦林和黄芩抑制草鱼呼肠孤病毒增殖的作用显著,板蓝根抑制病毒增殖的作用不明显;在药物与病毒共孵育后感染试验组中,利巴韦林对草鱼呼肠孤病毒有一定的直接杀灭作用,黄芩、板蓝根和穿琥宁等的体外杀病毒效果不明显。该模型的建立确认了药物在CIK细胞上具有抑制草鱼呼肠孤病毒的作用,不同给药方式药物的作用效果不同,也为抗病毒药物的筛选提供了有效的技术平台。
Using grass carp kidney cell line (CIK) and grass carp reovirus as materials, the cells were infected by the first administration with thiazolyl blue colorimetric assay, and then were administered after the first infection. The co- Screening of the virus-specific drugs, a in vitro anti-grass carp reovirus drug screening cell model. The results of the experimental group after first administration indicated that chuanhuning had the strongest effect in blocking the adsorption and invasion of cells of grass carp reovirus, followed by ribavirin and radix isatidis, and the scutellaria baicalin had the weakest blocking effect; while the first infection In the post-administration test group, chuanhuning, ribavirin and baicalin significantly inhibited the proliferation of grass carp reovirus, and the effect of Radix Isatidis on inhibiting the proliferation of the virus was not obvious. In the infected experimental group after drug and virus co-incubation, Lin grass carp reovirus has a direct killing effect, skullcap, Radix and chuanhuning and other in vitro killing virus effect is not obvious. The establishment of this model confirms that the drug has the inhibitory effect on grass carp reovirus on CIK cells. The effect of different drug delivery methods is different, which also provides an effective technology platform for the screening of antiviral drugs.