论文部分内容阅读
目的研究肛门直肠畸形(ARM)患儿直肠末端c-kit和SCF的表达水平,以探讨ARM患儿术后便秘的分子机制。方法通过RT-PCR方法研究10例高位、10例中位、8例低位ARM畸形和2例后天性肛瘘直肠末端c-kit mRNA和SCF mRNA的表达水平,并与正常对照组进行比较。结果与正常对照组相比较,c-kit mRNA、SCF mRNA的表达在高位ARM组、中位ARM组差异有显著意义(P＜0．05),后天性肛瘘组、低位ARM组差异无显著意义(P＞0．05)。结论低位ARM和后天性肛瘘直肠末端c-kit mRNA和SCF mRNA表达未见明显异常提示这两种疾病可能不影响患儿直肠末端Cajal间质细胞的发育和功能。高、中位ARM患儿直肠末端c-kit mRNA和SCF mRNA表达的减少可能是先天性的,将影响Cajal间质细胞的发育和功能,可能是患儿术后便秘产生的分子生物学证据之一。
Objective To study the expression of c-kit and SCF in the rectum of children with anorectal malformation (ARM) to explore the molecular mechanism of postoperative constipation in children with ARM. Methods The expression of c-kit mRNA and SCF mRNA in 10 cases of high, 10 cases of median, 8 cases of low ARM deformity and 2 cases of anal fistula were studied by RT-PCR and compared with the normal control group. Results Compared with the normal control group, the expression of c-kit mRNA and SCF mRNA in the high ARM group and the median ARM group were significantly different (P <0.05). There was no significant difference in the posterior fistula group and the low ARM group (P> 0.05). Conclusions There is no obvious abnormality of rectal c-kit mRNA and SCF mRNA expression in low ARM and acquired anal fistula, which suggests that these two diseases may not affect the development and function of rectum Cajal stromal cells in children. The reduction of c-kit mRNA and SCF mRNA expression in the rectum of high and middle ARM children may be congenital and will affect the development and function of Cajal interstitial cells, which may be the molecular biological evidence of postoperative constipation one.