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目的应用组织学的方法研究不同浓度壬基酚对泥鳅的肝脏毒性侵蚀程度。方法分别用50、200、500、1 000μg/L壬基酚溶液处理泥鳅,每隔6 h后观察其活动状态和体表特征,同时在每个容器中取1条泥鳅进行解剖,取其肝脏制片,观察肝细胞的胞质和细胞核情况,每隔6 h再取1条,继续观察。结果染色质浓集,边集并可见染色质呈环状或新月状附着核膜周边。结论随着壬基酚溶液浓度的增加,壬基酚对肝脏组织的毒性侵害越严重,对细胞核也会产生严重的毒害作用。
Objective To study the hepatotoxicity of nonylphenol on loach by using histological method. Methods Loach was treated with 50, 200, 500, and 1,000 μg / L nonylphenol solution respectively. The activity and body surface characteristics were observed every 6 h. At the same time, one loach was taken from each container for anatomy and its liver Production, observation of hepatocytes in the cytoplasm and nucleus, every 6 h and then take 1, continue to observe. Results Chromatin concentration, edge set and visible chromatin was ring or crescent attached nuclear perimeter. Conclusion With the increase of concentration of nonylphenol solution, nonylphenol is more toxic to liver tissue and more serious to the nucleus.