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体外培养的人喉癌细胞系Hep-2细胞用10 ng/ml干扰素-γ处理不同时间后,利用细胞计数、细胞凋亡-DNA Ladder分析及半定量RT-PCR方法,探讨干扰素-γ对Hep-2细胞增殖及凋亡的影响并初步分析其作用分子机制。结果显示,与不处理对照细胞相比,干扰素-γ处理后第2天起Hep-2细胞增殖明显变慢;细胞凋亡分析显示,干扰素-γ处理后Hep-2细胞基因组DNA电泳图谱呈梯状分布;半定量RT-PCR分析显示,干扰素-γ处理诱导Hep-2细胞IFI16基因表达。结果表明,干扰素-γ抑制Hep-2细胞增殖诱导Hep-2细胞凋亡,其机制可能与干扰素-γ诱导IFI16基因表达有关。
The human laryngeal carcinoma cell line Hep-2 cells were treated with 10 ng / ml interferon-γ for different time. The cell counting, apoptosis-DNA Ladder analysis and semi-quantitative RT-PCR were used to investigate the effects of interferon- On Hep-2 cell proliferation and apoptosis and preliminary analysis of its molecular mechanism of action. The results showed that the proliferation of Hep-2 cells was significantly slower than that of the untreated control cells on day 2 after IFN-γ treatment. The results of apoptosis analysis showed that the genomic DNA of Hep-2 cells treated with IFN- Showed a ladder-like distribution; semi-quantitative RT-PCR analysis showed that interferon-γ treatment induced IFI16 gene expression in Hep-2 cells. The results showed that interferon-γ could inhibit the proliferation of Hep-2 cells and induce the apoptosis of Hep-2 cells. The mechanism may be related to IFN-γ-induced IFI16 gene expression.