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目的:观察三七总皂苷对急性心肌梗死(acute myocardial infarction,AMI)大鼠骨髓干细胞(bone marrow stemcells,BMSC)动员的效率。方法:140只Wistar大鼠建立急性AMI模型后随机分成:西药动员组(皮下注射G-CSF50μg.kg-1.d-1;假手术组和模型组皮下注射生理盐水50μg.kg-1.d-1;中药动员组腹腔注射血塞通150 mg.kg-1.d-1;中西药动员组皮下注射G-CSF50μg.kg-1.d-1和腹腔注射血塞通150 mg.kg-1.d-1,除假手术组外,各组又按1,7,14 d 3个时间点分为3个亚组,其中G-CSF每天1次,共用7 d,血塞通注射液每天1次,直到每亚组处死动物前停止,采用流式细胞仪检测外周血中C-kit+细胞量和全自动血细胞分离机观察白细胞(white blood cell,WBC)计数和MNC(mononuclear cell,MNC)百分比。结果:模型组与假手术组1,7,14 d比较WBC、MNC%明显升高(P<0.05);在1,7,14 d中药组、西医药组与同期中西医药组相比明显低(P<0.05);西药组、中西医药组与模型组相比外周血单个核细胞C-kit受体表达明显升高(P<0.05),中西药组与各组相比变化明显。结论:三七总皂苷可协同G-CSF更好动员BMSC,提高BMSC出髓效率,增加外周血中BMSC数量。
OBJECTIVE: To observe the efficiency of Panax notoginseng saponins in the mobilization of bone marrow stem cells (BMSCs) in rats with acute myocardial infarction (AMI). METHODS: One hundred and forty Wistar rats were randomly divided into two groups: the western medicine mobilization group (subcutaneous injection of G-CSF 50μg.kg-1.d-1); the sham group and model group were injected subcutaneously with saline 50μg.kg-1.d. -1; Chinese medicine mobilization group intraperitoneal injection Xuesaitong 150 mg.kg-1.d-1; Chinese and Western medicine mobilization group subcutaneous injection of G-CSF 50μg.kg-1.d-1 and intraperitoneal injection of Xuesaitong 150 mg.kg- 1.d-1, in addition to the sham group, each group was divided into three subgroups according to 1, 7 and 14 days, including G-CSF once a day, sharing 7 days, Xuesaitong injection Once a day until each subgroup was sacrificed before the animals were sacrificed, flow cytometry was used to measure the amount of C-kit+ cells in peripheral blood and automatic blood cell separator was used to observe white blood cell (WBC) counts and MNC (mononuclear cell, MNC). ) Percentage.Results: WBC and MNC% were significantly higher in model group and sham operation group on days 1, 7, and 14 (P<0.05); in 1, 7 and 14 days, traditional Chinese medicine group, western medicine group and the same period of Chinese and Western medicine group. The ratio was significantly lower (P<0.05); Western medicine group, Chinese and Western medicine group compared with the model group C-kit receptor expression in peripheral blood mononuclear cells was significantly higher (P<0.05), Chinese and Western medicine group compared with the groups significantly Conclusion: Panax notoginseng saponins can work better with G-CSF Members BMSC, BMSC improve the efficiency marrow, increasing the number of peripheral blood BMSC.