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目的:研究齐藤凹顶藻Laurencia saitoi化学成分,并对单体化合物进行细胞毒活性筛选。方法:采用正相硅胶柱色谱、Sephadex LH-20凝胶色谱、重结晶等方法进行分离纯化;应用MS,1D和2D NMR等波谱学方法鉴定结构;通过MTT法对单体化合物进行细胞毒活性测试。结果:从齐藤凹顶藻中分离并鉴定了7个化合物,分别为aplysistatin(1),5-acetoxy-palisadinB(2),palisadin B(3),palisadin A(4),pacifigorgiol(5),豆甾-4-烯-3α,6β-二醇(6),2,3,5,6-四溴-吲哚(7),其中化合物1～5为倍半萜类化合物,化合物6为甾醇类化合物,化合物7为四溴取代的吲哚类化合物。对化合物在人肿瘤细胞株Bel-7402,BGc-823模型上进行了细胞毒活性测定,IC50均大于10μg.mL-1。结论:所有化合物均为首次从该种海藻中分离得到,但对人肿瘤细胞株Bel-7402,BGc-823均无明显活性。
Objective: To study the chemical constituents of Laurencia saitoi and screen the cytotoxic activity of the monomeric compounds. Methods: The compounds were isolated and purified by normal phase silica gel column chromatography, Sephadex LH-20 gel chromatography and recrystallization. The structures were identified by MS, 1D and 2D NMR spectroscopic methods; the cytotoxic activity of the monomeric compounds was determined by MTT assay. test. RESULTS: Seven compounds were isolated and identified from A. vulgaris, aplysistatin(1), 5-acetoxy-palisadinB(2), palisadin B(3), palisadin A(4), pacifigorgiol(5). Soybean-4-ene-3α,6β-diol (6), 2,3,5,6-tetrabromo-indene (7), wherein compounds 1 to 5 are sesquiterpene compounds, and compound 6 is sterol The compound, Compound 7, is a tetrabromo-substituted terpenoid. The compounds were tested for cytotoxicity on human tumor cell lines Bel-7402 and BGc-823 models, and the IC50 values were all greater than 10 μg.mL-1. Conclusion: All compounds were isolated from the seaweed for the first time, but no significant activity was observed in human tumor cell lines Bel-7402 and BGc-823.