钩虫蚴虫培养方法诸多。笔者采用双玻皿滤纸炭末培养法,培养活体钩蚴,应用于实验数学及标本制作中,获得了较为满意的效果。现将该方法报道如下。一、虫卵收集取钩虫阳性粪便,用沉淀法集卵。二、材料准备 1.炭末:取木炭一小块,用研钵研碎之,以60～80目/时筛,筛取炭末,装瓶置于140℃,二小时干燥消毒。2.玻皿:以直径15cm玻皿一只作为“底皿”。直径12cm玻皿一只作为“上皿”。上皿底朝上,其上复盖二层直径20cm的普通滤纸,滤纸边缘部分包裹玻皿周边并向其壁反折。为使滤纸反折部 Hookworm Cercariae worm culture many ways. The author uses double glass filter paper carbon powder culture method to cultivate live hook larvae, used in experimental math and specimen production, obtained more satisfactory results. The method is reported below. First, the eggs collected hookworm positive stool, precipitation method set eggs. Second, the material preparation 1 charcoal: Take a small piece of charcoal, ground with a mortar to 60 ~ 80 mesh / hour sieve, screened charcoal, bottled placed at 140 ℃, two hours of dry disinfection. 2. glass dish: 15cm in diameter glass dish as a “dish.” A glass bowl 12cm diameter as a “dish.” The bottom of the dish up, covered with two layers of ordinary diameter 20cm filter paper, the edge of the filter paper wrapped around the glass and its back to the wall. To make the filter paper folding section