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目的探讨异基因小鼠髓腔内骨髓移植(IBM-BMT)诱导免疫耐受的效果。方法雄性BALB/c小鼠和雌性C57BL/6小鼠分别作为骨髓移植的供、受者。受者预处理后进行IBM-BMT,建立异基因小鼠骨髓移植模型。通过皮肤移植和混合淋巴细胞反应(MLR)对受者的耐受状态进行检测。结果接受过IBM-BMT的受者进行供者来源的皮肤移植,移植物的存活时间>300d,较对照组的(12.7±1.63)d明显延长(P<0.01),而受者接受来自无关供者(KM小鼠)的皮肤移植物存活时间未见延长。接受过IBM-BMT的受者脾细胞对供者脾细胞的MLR增殖率均明显降低,与对照组比较,P<0.01,而对无关供者的脾细胞仍表现强烈的增殖反应。结论应用IBM-BMT可以诱导受者获得供者抗原的特异性免疫耐受,使移植物存活时间延长。
Objective To investigate the effect of allogeneic mouse bone marrow transplantation (IBM-BMT) induced immune tolerance. Methods Male BALB / c mice and female C57BL / 6 mice were used as recipients and recipients of bone marrow transplantation respectively. The recipients pretreated with IBM-BMT to establish a allogeneic mouse bone marrow transplantation model. The recipient’s tolerance status was tested by skin graft and mixed lymphocyte reaction (MLR). Results The recipients of IBM-BMT received skin grafts from donor donors. The graft survival time was> 300d, significantly longer than that of the control group (12.7 ± 1.63) d (P <0.01) (KM mice) had no prolonged graft survival. The proliferation rate of donor splenocytes from splenocytes of recipients who received IBM-BMT was significantly lower than that of the control group (P <0.01), while splenocytes from unrelated donors still showed strong proliferative responses. Conclusion The application of IBM-BMT can induce recipients to obtain specific immune tolerance of donor antigens, prolonging graft survival.