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目的探讨大剂量甲基强的松龙(MP)对AQP4表达的影响及其对实验性脊髓空洞前状态脊髓水肿的保护机制。方法选用新西兰白兔,制作动物模型并应用大剂量MP干预,采用干湿重法测定脊髓含水量,用免疫组化、Western blot和RT-PCR技术检测脊髓空洞前状态AQP4及其mRNA表达变化。结果Kaolin组动物于术后第1天脊髓含水量即有明显增加,第7天达到高峰, 21d时稍有缓解;AQP4于术后第1天开始减弱,第7-14天达到最低水平,到21d时回升;而AQP4 mRNA表达变化趋势与其蛋白含量变化相一致。MP组动物脊髓水肿时间延迟,程度显著减轻,上颈髓AQP4及其mRNA明显增高,以7d、14d最为显著。结论MP能够在基因和蛋白水平增加AQP4表达,促进水分子向血管腔和蛛网膜下腔转运,发挥抑制脊髓水肿作用。
Objective To investigate the effect of high-dose methylprednisolone (MP) on the expression of AQP4 and its protective mechanism on spinal cord edema before experimental cavitation. Methods New Zealand white rabbits were selected to make animal model and high-dose MP intervention. The spinal cord water content was measured by wet-dry method. The expression of AQP4 and its mRNA in pre-caval cavity was detected by immunohistochemistry, Western blot and RT-PCR. Results In the Kaolin group, the spinal cord water content increased significantly on the first postoperative day, reaching the peak on the seventh day and relieving slightly on the 21st day. The AQP4 began to weaken on the first postoperative day and reached the lowest level on the 7-14th day. 21d, and the trend of AQP4 mRNA expression was consistent with that of its protein content. The time of spinal edema in MP group was delayed, and the level of AQP4 and its mRNA in the cervical spinal cord was significantly increased, which was the most significant at 7d and 14d. Conclusion MP can increase the expression of AQP4 at the gene and protein levels, promote the transport of water molecules to the blood vessel and subarachnoid space, and exert the inhibitory effect on spinal cord edema.