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目的:探讨人乳头瘤病毒(HPV16)感染及端粒酶激活与维吾尔族子宫颈癌的关系。方法:对32例维吾尔族宫颈癌、25例宫颈上皮内瘤变(CIN)及33例慢性宫颈炎患者应用PCR(聚合酶链反应)技术及PCR-TRAP(重复序列扩增)法进行HPV16及端粒酶活性检测。结果:(1)端粒酶阳性表达率依次为:CIN>宫颈癌>慢性宫颈炎;CINⅢ级>CINⅠ、Ⅱ级(P<0.05)。端粒酶活性水平:CIN>宫颈癌>慢性宫颈炎(P<0.05);CINⅢ级>CINⅠ、Ⅱ级(P<0.05)。(2)HPV16阳性表达率依次为:宫颈癌>CIN>慢性宫颈炎(P<0.05)。(3)宫颈组织中HPV16及端粒酶协同阳性表达率为:宫颈癌>CIN>慢性宫颈炎(P<0.05);(4)HPV16及端粒酶在宫颈癌早期与晚期、高分化与中低分化组织中表达差异均无统计学意义,在宫颈鳞癌与腺癌中的表达差异无统计学意义(P>0.05)。结论:HPV16感染和端粒酶激活与维吾尔族子宫颈癌的发生密切相关,端粒酶激活是宫颈癌发生的早期事件。
Objective: To investigate the relationship between human papillomavirus (HPV16) infection and telomerase activation and Uygur cervical cancer. Methods: 32 cases of Uigur cervical cancer, 25 cases of cervical intraepithelial neoplasia (CIN) and 33 cases of chronic cervicitis were studied by PCR (polymerase chain reaction) and PCR-TRAP (repeat amplification) Detection of telomerase activity. Results: (1) The positive rates of telomerase were CIN> cervical cancer> chronic cervicitis; CIN Ⅲ> CIN Ⅰ, Ⅱ (P <0.05). The levels of telomerase activity: CIN> cervical cancer> chronic cervicitis (P <0.05); CIN Ⅲ> CIN Ⅰ, Ⅱ (P <0.05). (2) The positive rates of HPV16 were as follows: cervical cancer> CIN> chronic cervicitis (P <0.05). (3) The positive rates of HPV16 and telomerase expression in cervical tissues were: cervical cancer> CIN> chronic cervicitis (P <0.05); (4) The expression of HPV16 and telomerase in early and late stage of cervical cancer, There was no significant difference in the expression between poorly differentiated tissues and cervical squamous cell carcinoma and adenocarcinoma (P> 0.05). Conclusion: HPV16 infection and telomerase activation are closely related to the occurrence of Uighur cervical cancer. Telomerase activation is an early event in cervical cancer.