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目的 研究人IL 2、IFN γ基因修饰对肝癌、胆管癌细胞株免疫原性的影响。方法 构建携带人IL 2、IFN γcDNA的逆转录病毒表达载体 ,导入人肝癌HepG2或胆管癌QBC939细胞系 ,运用WesternBlot和ELISA法分析导入IFN γ基因的靶细胞MHCⅠ类分子的表达 ,淋巴细胞杀伤试验和肿瘤细胞在裸鼠体内的成瘤性改变 ,研究导入目的基因的肿瘤细胞免疫原性的变化。结果 WesternBlot和ELISA分析 ,证明导入IFN γ基因后瘤细胞MHCⅠ类分子表达水平增加 ,并能增强HLAA2位点相匹配肝癌、胆管癌病人外周血淋巴细胞杀伤亲本瘤细胞的活性 ;导入IL 2基因瘤细胞在裸鼠体内成瘤性降低。结论 IL 2和IFN γ基因修饰能增强肝癌HepG2、胆管癌QBC939细胞系的免疫原性。
Objective To study the effect of human IL 2 and IFN γ gene modification on the immunogenicity of hepatocellular carcinoma and cholangiocarcinoma cell lines. Methods The retroviral expression vector carrying human IL 2 and IFN γ cDNA was constructed and introduced into human hepatocellular carcinoma HepG2 or cholangiocarcinoma cell line QBC939. Western blot and ELISA were used to analyze the expression of MHC class I molecules in the target cells transfected with IFNγ gene. The tumorigenicity of tumor cells in nude mice was studied, and changes in the immunogenicity of the tumor cells into which the gene of interest was introduced were studied. RESULTS: Western blot and ELISA analysis showed that the expression of MHC class I molecules in tumor cells was increased after the introduction of IFN gamma gene, and the activity of HLA A2 locus matching lymphocytes from peripheral blood of patients with hepatocellular carcinoma and cholangiocarcinoma to kill parental tumor cells was enhanced; IL 2 gene tumor was introduced. The tumor cells in the nude mice have reduced tumorigenicity. Conclusions IL 2 and IFN γ gene modification can enhance the immunogenicity of hepatocellular carcinoma HepG2 and cholangiocarcinoma QBC939 cell lines.