论文部分内容阅读
目的研究莪术油对中波紫外线所致皮肤损伤的防护作用及其机制。方法建立UVB氧化损伤模型(照射30d,累计28·38J/cm2)。观察皮肤外观及HE染色后表皮厚度和真皮成纤维细胞数量的变化。酶法检测皮肤匀浆上清液中抗氧化酶(过氧化氢酶CAT、谷胱甘肽GSH-Px和超氧化物歧化酶SOD)活性和丙二醛(MDA)含量及总抗氧化能力(T-AOC)。结果①光镜下,UVB照射组和基质空白组照射侧可见表皮增厚,真皮中成纤维细胞减少,而对照侧未见此病理变化;莪术油防护组照射侧表皮未见增厚,成纤维细胞数增多,但对照侧成纤维细胞数增多不明显。②UVB照射组和基质空白组照射侧皮肤中MDA含量明显升高,CAT、GSH-Px和SOD活性及T-AOC降低,而对照侧未见明显差异;莪术油防护组照射侧CAT、GSH-Px、SOD活性及T-AOC增强,MDA含量降低,而对照侧未见明显改变。结论莪术油具有对UVB皮肤氧化损伤的防护作用,其作用机制可能与提高抗氧化酶含量及清除体内自由基有关。
Objective To study the protective effect of zedoary oil on the skin damage caused by UVB and its mechanism. Methods A UVB oxidative injury model was established (30 days irradiation, cumulative 28.38 J/cm2). The appearance of the skin and changes in the thickness of the epidermis and the number of dermal fibroblasts after HE staining were observed. The activity of antioxidant enzymes (catalase CAT, glutathione GSH-Px, and superoxide dismutase SOD), malondialdehyde (MDA) content and total antioxidant capacity were measured by enzymatic method in the supernatant of skin homogenate. T-AOC). Results 1 Under the light microscope, the epidermis thickened and the fibroblasts in the dermis decreased in the UVB irradiation group and the matrix blank group, but no pathological changes were found in the control side; no thickening and fibroblasts were seen in the irradiated side of the Curcuma oil protective group. The number of cells increased, but the number of fibroblasts on the control side did not increase significantly. MDA content in irradiated skin of 2UVB irradiation group and matrix blank group was significantly increased, CAT, GSH-Px and SOD activity and T-AOC were decreased, while no significant difference was found on the control side; CAT, GSH-Px on the irradiation side of Curcuma oil protection group , SOD activity and T-AOC enhancement, MDA content decreased, but no significant changes in the control side. Conclusion Zedoary oil has the protective effect against UVB skin oxidative damage. Its mechanism may be related to the increase of antioxidant enzyme content and clearance of free radicals in vivo.