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目的建立记录觉醒大鼠听觉皮层神经元细胞外电活动的方法。方法利用自制的大鼠头部固定器,结合大鼠固定训练,对觉醒大鼠进行有效的固定,记录听觉皮层神经元对声音的反应。结果成功记录到觉醒大鼠听觉皮层单神经元对声音刺激的反应。结论该方法成熟稳定,可应用于觉醒大鼠听觉神经学研究。
Objective To establish a method for recording the extracellular electrical activity of the auditory cortex neurons in awakening rats. Methods Self-made rat head immobilizer was used in combination with rat fixed training to effectively immobilize the awakened rats and record the auditory cortex neurons response to the sound. Results The auditory cortex mononeurons of awake rats were successfully recorded in response to sound stimulation. Conclusion The method is mature and stable and can be applied to the auditory neurology of awake rats.