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研究鼻咽癌、肺癌与正常鼻咽组织基因表达谱差异及筛选鼻咽癌相关基因, 采用α32P逆转录标记组织总RNA, 将cDNA 探针与有5 184 个基因或表达序列标签EST (expression sequence tag) 的高密度cDNA 微阵列GF200 杂交, 软件分析表达谱差异. 结果发现三者均呈低表达为主的表达谱, 密度值在200 以上的基因及EST在鼻咽癌有110 个, 肺癌134 个而鼻咽组织有158 个; 5 个EST在鼻咽高表达但鼻咽癌低表达,3 个EST在鼻咽癌高表达但正常鼻咽低表达. 结果表明鼻咽癌与正常鼻咽及肺癌组织存在差异表达基因, 可能还有新基因在鼻咽癌发生中起作用; 采用高密度cDNA微阵列是一种筛选差异表达基因的快速有效方法
To study the difference of gene expression between nasopharyngeal carcinoma, lung cancer and normal nasopharyngeal tissues and to screen the related genes of nasopharyngeal carcinoma. Total RNA was labeled by α32P reverse transcription, and cDNA probes were compared with 5 184 genes or EST expression sequence tag) high-density cDNA microarray GF200 hybridization, the software analysis of expression differences. The results showed that all the three genes were expressed in low expression level. The genes and ESTs with the density of more than 200 were 110 in nasopharyngeal carcinoma, 134 in lung cancer and 158 in nasopharyngeal tissue. The five ESTs were highly expressed in nasopharynx However, low expression of nasopharyngeal carcinoma, three ESTs in nasopharyngeal carcinoma but normal expression of low nasopharyngeal. The results showed that nasopharyngeal carcinoma and normal nasopharyngeal and lung cancer tissue differentially expressed genes, and may have new genes play a role in the occurrence of nasopharyngeal carcinoma; using high-density cDNA microarray is a screening of differentially expressed genes in a quick and effective method