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以弓形虫的玻片为抗原，进行了免疫酶染色试验（ＩＥＳＴ），检测２０份阳性兔血清和５２份阳性人血清，阳性率分别为１００％和９６．１５％；２０份阴性兔血清和１９３份阴性人血清，假阳性率为０和１．０４％；此法与血吸虫病、肝吸虫病及疟疾均无交叉反应，说明对弓形虫有较高的特异性；用该法对精神病患者、孕妇及健康人群进行了弓形虫感染的检测，其结果分别为２４．５１％、１３．３５％和７．３２％，此结果与染色试验（ＤＴ）无显著性差异。ＩＥＳＴ法具有简便、经济、敏感、特异等优点，适用于现场推广应用。
Toxoplasma gondii slides were used as antigen to perform immunoenzyme staining (IEST). The positive rates of positive serum were 100% and 96.15% respectively in 20 positive rabbit serum and 52 positive human serum. Twenty rabbits serum and 193 negative human serum, the false positive rate was 0 and 1.04%; this method and schistosomiasis, hepaticosis and malaria no cross-reaction, indicating that Toxoplasma gondii has a higher specificity; the use of the method of mental illness , Pregnant women and healthy population of Toxoplasma gondii infection, the results were 24.51%, 13.35% and 7.32%, the results and staining test (DT) no significant difference. IEST method is simple, economical, sensitive, specific and other advantages, suitable for on-site promotion and application.