酶联免疫吸附试验（Ｅｎｚｙｍｅ　Ｌｉｎｋｅｄ　Ｉｍｍｕｎｏｓｏｒ－ｂｅｎｔ　Ａｓｓａｙ，ＥＬＩＳＡ）于７０年代初出现，是酶免疫测定技术的一种，也是其在方法上的一个重大改进，用于测定液相内的微量蛋白质，近年来不断改进，还可用于测定其他微量物质。以固一液抗原 Enzyme Linked Immunosorbent Assay (ELISA) appeared in the early 70s, which is a kind of enzyme immunoassay technique and a significant improvement on the method. It is used to measure the trace protein in the liquid phase, In recent years, continuous improvement, but also can be used for the determination of other trace substances. To solid-liquid antigen