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以细胞核分裂观察、细胞周期实验以及有害自由基测定,N-亚硝基吗啉的形成及含量测定为观察指标,研究了福建银芝 YZ08乌龙茶对体外培养人胃腺癌细胞 MGC-803的抗肿瘤作用。结果表明,YZ08 乌龙茶能有效抑制胃癌细胞的核分裂,对细胞的分化阻断效应发生在细胞增殖分化早期阶段,即 DNA 合成前期。YZ08乌龙茶能明显的清除生物大分子辐射损伤产生的有害自由基,对亚硝基吗啉合成具有阻断作用。上述实验表明,YZ08乌龙茶对肿瘤具有良好的化学预防作用。作者曾报道银芝 YX08乌龙茶对体外培养的人胃癌细胞生长有明显的抑制作用,本实验旨在进一步观察 YZ08乌龙茶对胃癌细胞核分裂,细胞周期以及对有害自由基的清除和阻断亚硝基吗啉合成作用,,从基础理论上证明 YZ08乌龙茶的防癌功效。
The observation of cell nuclear division, cell cycle assay, and harmful free radicals, the formation and content determination of N-nitroso-morpholine were observed, and the anti-tumor effect of Fujian Yinzhi YZ08 oolong tea on human gastric cancer cell MGC-803 was studied. effect. The results showed that YZ08 oolong tea can effectively inhibit the nuclear fission of gastric cancer cells, and the effect on cell differentiation and differentiation occurs in the early stage of cell proliferation and differentiation, ie, the early stage of DNA synthesis. YZ08 oolong tea can obviously remove harmful free radicals produced by radiation damage of biological macromolecules and has a blocking effect on the synthesis of nitroso morpholine. The above experiments show that YZ08 oolong tea has a good chemopreventive effect on tumors. The authors have reported that Yinzhi YX08 oolong tea has a significant inhibitory effect on the growth of human gastric cancer cells cultured in vitro. This experiment aims to further observe the effect of YZ08 oolong tea on gastric cancer cell nuclear division, cell cycle, and removal of harmful free radicals and block nitroso. The role of morpholine synthesis, from the basic theoretical proof of the anti-cancer efficacy of YZ08 oolong tea.