人胚胎干细胞具有多向分化潜能,由人胚胎干细胞(human embryonic stem cells,hESCs)诱导分化的各种目的细胞产生的阶段性成果,为hESCs的临床应用积累了丰富的实验数据,并为细胞移植治疗临床疾病奠定了基础。内胚层细胞由于其构成器官的复杂性和hESCs诱导分化的难度及临床迫切需要的矛盾尚未解决,仍然是hESCs诱导分化方向的研究热点。本文对hESCs向内胚层细胞诱导分化的方式、信号通路、研究成果、问题及展望等作一综述。 Human embryonic stem cells have multi-directional differentiation potential, and the phased results produced by various target cells induced by human embryonic stem cells (hESCs) accumulate abundant experimental data for the clinical application of hESCs, Laid the foundation for the treatment of clinical diseases. Endodermal cells due to its complexity of organ formation and differentiation of hESCs difficult and the urgent need for clinical contradictions have not yet been resolved, is still hESCs differentiation direction of the research focus. In this paper, we summarize the ways and signaling pathways of hESCs differentiation into endoderm cells, the research results, problems and prospects.