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以不同浓度的氚化氨基酸(包括〔~3H〕精氨酸、〔~3H〕赖氨酸、〔~3H〕亮氨酸和〔~3H〕天冬氨酸)和氚化胸腺核苷处理培养中的小鼠淋巴白血病(L5178Y)对数生长期细胞50小时,测量氚的活性并以每个细胞或细胞核中的~3H衰变数表示。测量细胞与细胞核的直径以计算其体积。每个细胞或细胞核的累积衰变值可以从dpm/细胞(dpm/细胞核)与在有放射活性培养基中孵育时间,及dpm/细胞(dpm/细胞核)与在无放射活性培养基中照射后培养时间的关系曲线下的面积而获得。计算细胞及细胞核的平均吸收剂量。细胞存活资料适合公式S=eXp(-αD),突变资料适合公式Y=αD,D为总
Different concentrations of deuterated amino acids (including [~3H]arginine, [~3H]lysine, [~3H]leucine and [~3H]aspartic acid) and deuterated thymidine treatment In mouse lymphoid leukemia (L5178Y), the cells in the growth phase were exposed for 50 hours, and the activity of the sputum was measured and expressed as the number of ~3H decays per cell or cell nucleus. The diameters of cells and nuclei are measured to calculate their volume. The cumulative decay value of each cell or cell nuclei can be cultured from dpm/cell (dpm/nucleus) and incubation time in radioactive medium, and dpm/cell (dpm/nucleus) and irradiated in non-radioactive medium. Time is obtained by the area under the curve. Calculate the average absorbed dose of cells and nuclei. The cell survival data is suitable for the formula S=eXp(-αD), the mutation data is suitable for the formula Y=αD, D is the total