AIM:To determine the regulatory effects of recombinanthuman growth hormone(rhGH)on dipeptide transport(PepT1)in normal and severe scald rats.METHODS:Male Sprague-Dawley rats with 30 % totalbody surface area(TBSA)Ⅲ degree scald were employedas the model.In this study rhGH was used at the dose of2 IU.kg~(-1)d~(-1).An everted sleeve of intestine 4 cm long obtainedfrom mid-jejunum was securely incubated in Kreb’s solutionwith radioactive dipeptide(~3H-glycylsarcosine,~3H-Gly-Sar,10 μCi/ml)at 37 ℃ for 15 min to measure the effects ofuptake and transport of PepT1 of small intestinal epithelialcells in normal and severe scald rats.RESULTS:Abundant blood supply to intestine and mesenterywas observed in normal and scald rats administered rhGH,while less supply of blood to intestine and mesentery wasobserved in rats without rhGH.Compared with controls,thetransport of dipeptide in normal rats with injection of rhGHwas not significantly increased(P=0.1926),while the uptakewas significantly increased(P=0.0253).The effects oftransport and uptake of PepT1 in scald rats with injection ofrhGH were significantly increased(P 0.0082,0.0391).CONCLUSION: Blood supply to intestine and mesentery of rats was increased following injection of rhGH. The effects of uptake and transport of dipeptide transporters in small intestinal epithelial cells of rats with severe scald were markedly up-regulated by rhGH. AIM: To determine the regulatory effects of recombinant human growth hormone (rhGH) on dipeptide transport (PepT1) in normal and severe scald rats. METHODS: Male Sprague-Dawley rats with 30% totalbody surface area (TBSA) III degree scald were employedas the model .In this study rhGH was used at the dose of 2 IU.kg ~ (-1) d ~ (-1) .An everted sleeve of intestine 4 cm long obtainedfrom mid-jejunum was firmly incubated in Kreb’s solutionwith radioactive dipeptide (~ 3H- glycylsarcosine, ~ 3H-Gly-Sar, 10 μCi / ml) at 37 ° C for 15 min to measure the effects of uptake and transport of PepT1 of small intestinal epithelial cells in normal and severe scald rats. RESULTS: Abundant blood supply to intestine and mesenterywas observed in normal and scald rats administered rhGH, while less supply of blood to intestine and mesentery wasobserved in rats without rhGH. Compared with controls, the transport of dipeptide in normal rats with injection of rhGH was not significantly increased (P = 0.1926), while the uptake was significantly in The effects of transport and uptake of PepT1 in scald rats with injection of rhGH were significantly increased (P 0.0082, 0.0391). CONCLUSION: Blood supply to intestine and mesentery of rats was increased following injection of rhGH. The effects of uptake and transport of dipeptide transporters in small intestinal epithelial cells of rats with severe scald were markedly up-regulated by rhGH.