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目的探讨细丝蛋白A(filamin A,FLNa)在胃癌组织中的表达水平及其临床意义。方法本研究应用逆转录聚合酶链反应(RT-PCR)和免疫组织化学方法(SP法),检测47例胃癌组织,及距胃癌组织边缘5 cm以上的显微镜下无癌浸润的正常组织中mRNA及蛋白的表达情况。结果 FLNa蛋白和mRNA在胃癌组织及正常胃组织中的表达阳性率分别为0.345±0.048、0.562±0.085和29.8%、78.7%,较正常胃组织明显降低(P<0.05)。结论胃癌组织中FLNamRNA和蛋白的表达较正常组织均降低,这可能与胃癌的发生、发展有关。因此,检测FLNa的表达可能对胃癌的临床治疗及预后判断有一定价值。
Objective To investigate the expression of filamin A (FLNa) in gastric carcinoma and its clinical significance. Methods In this study, we detected the expression of mRNA in 47 cases of gastric cancer tissues and in normal tissues without cancer invasion under the microscope more than 5 cm away from the edge of gastric cancer tissues by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry (SP method) And protein expression. Results The positive rates of FLNa protein and mRNA expression in gastric cancer tissues and normal gastric tissues were 0.345 ± 0.048, 0.562 ± 0.085 and 29.8%, respectively, 78.7%, which were significantly lower than those in normal gastric tissues (P <0.05). Conclusion The expression of FLNamRNA and protein in gastric cancer tissues are lower than that in normal tissues, which may be related to the occurrence and development of gastric cancer. Therefore, the detection of FLNa expression may have some value in the clinical treatment and prognosis of gastric cancer.