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紫外差示光谱法是测定生物检材中巴比妥类药物含量的常用方法[1]，但该法在进行检材中的药物分离所采用的液液萃取，操作繁琐。采用固相革取分离脏器中巴比妥类药物，操作简便。由于革取液中含有一定量的杂质，对紫外差示光谱法测定有较明显的干扰，故用紫外差示导数光谱法测定
UV differential spectrometry is a commonly used method for the determination of barbiturates in biological samples [1], but this method is complicated to carry out by liquid-liquid extraction used for drug separation in samples. The use of solid phase separation of organs in the barbiturates drugs, easy to operate. As a result of the extract contains a certain amount of impurities on the UV differential spectrometry determination of a more obvious interference, it is determined by UV differential spectroscopy