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作者介绍一种用酶联免疫吸附测定法(ELISA)测定病毒蛋白从而测定干扰素(IFN)抗病毒活性的方法。测定时酶结合物可用蛋白A、抗免IgG或抗滤泡性口炎病毒(VSV)的碱性磷酸酶结合物。结合方法为二步法,即首先将碱性磷酸酶在PBS中透析,用戊二醛活化,经再次透析后,加入蛋白A或抗VSV使之结合,最后用Sephacryl S-300型柱分离结合物。羊抗兔IgG碱性磷酸酶结合物系Tago或Sigma商品。首先用细胞生长培养基将IFN样品二倍系列稀
The authors describe a method for the determination of antiviral activity of interferon (IFN) by measuring viral proteins by enzyme-linked immunosorbent assay (ELISA). Enzyme conjugates are assayed for protein A, anti-IgG, or alkaline phosphatase conjugate against follicular stomatitis virus (VSV). The combination method is a two-step method, that is, firstly, alkaline phosphatase is dialyzed in PBS, activated with glutaraldehyde, and after dialysis again, protein A or anti-VSV is added for binding, finally, Sephacryl S-300 type column is used for separation and binding Things. Goat anti-rabbit IgG alkaline phosphatase conjugate is a Tago or Sigma product. IFN samples were first diluted twice with cell growth medium