论文部分内容阅读
目的 :为了解睫状神经营养因子 ( CNTF)在大鼠生后心肌中的表达变化。方法 :用免疫组织化学法和计算机图像处理系统 ,研究 1天龄、1月龄、2月龄、3月龄、2年龄大鼠心肌中 CNTF免疫反应的变化。结果 :在各年龄组中均存在阳性免疫反应 ,阳性免疫反应见于心肌细胞膜上 ,各年龄组的平均灰度值分别为 2 5 .12± 0 .5 4、5 0 .12± 0 .6 7、5 7.2 5± 0 .6 2、43 .81± 0 .83、3 2 .14± 0 .73。结论 :睫状神经节因子在出生后的心肌细胞内即存在 ,其含量随心肌的发育而有所变化
Objective: To investigate the expression changes of ciliary neurotrophic factor (CNTF) in postnatal myocardium. Methods: Immunohistochemistry and computer image processing system were used to study the changes of CNTF immunoreactivity in 1-day, 1-month, 2-month, 3-month and 2-year old rats. Results: There were positive immune responses in all age groups, and the positive immunoreactivity was found in the myocardial cell membrane. The average gray values of all age groups were 25.12 ± 0.5, 4.5, 0.12 ± 0.67 , 5 7.2 5 ± 0. 6 2,43 .81 ± 0 .83,3 2 .14 ± 0 .73. CONCLUSION: Ciliary ganglion factor is present in postnatal cardiomyocytes and its content varies with the development of myocardium