论文部分内容阅读
通过观察αB-晶状体蛋白在骨骼肌细胞的变化,了解骨骼肌细胞抵抗运动损伤的机制。方法:成年雄性Wistar大鼠24只,随机分为安静对照组,运动后即刻组,运动后24 h组,每组8只,运动组进行一次性大强度离心运动;取腓肠肌制作冰冻切片,采用免疫荧光组织化学法观察αB-晶状体蛋白在骨骼肌细胞的分布特征。结果:安静状态αB-晶状体蛋白在细胞浆有一定水平的表达,主要分布于胞浆及膜下。离心运动后即刻αB-晶状体蛋白表达增加并且发生移位变化,主要存在于Z盘及细胞膜附近,24 h后αB-晶状体蛋白在细胞膜和Z盘仍有大量分布。结论:离心运动后αB-晶状体蛋白从肌细胞胞浆移位于细胞膜和Z盘,其在骨骼肌可能具有防止Z盘和肌细胞膜骨架蛋白损伤或辅助骨架蛋白修复的作用。
By observing the changes of αB-crystallin in skeletal muscle cells, we can understand the mechanism of skeletal muscle cell resistance to exercise injury. Methods: Twenty-four adult male Wistar rats were randomly divided into 4 groups: control group, exercise group, exercise group and exercise group at 24 h after exercise. The rats in exercise group were subjected to one-time high intensity centrifugation. Frozen sections were obtained from gastrocnemius muscle. Immunofluorescence histochemistry to observe the distribution of αB-crystallin in skeletal muscle cells. Results: In resting state, αB-crystallin was expressed in cytoplasm at a certain level, mainly distributed in the cytoplasm and under the membrane. After centrifugation, the expression of αB-crystallin increased and shifted, mainly in the vicinity of Z disk and cell membrane. After 24 h, there was still a large number of distribution of αB-crystallin in cell membrane and Z disk. CONCLUSION: αB-crystallin migrates from the cytoplasm of myocytes to the cell membrane and the Z-plate after eccentric exercise. It may play a role in skeletal muscle damage of the Z-plate and muscle cell membrane proteins or assist in the repair of scaffold proteins.