第三章愈伤组织的培养 3.1 历史十九世纪三十年代末期Gautheret和Nobecourt在欧洲,White在美国同时首创一种技术,革新了植物组织培养方法。1939年Gautheret报道把胡萝卜根的外植体放在含有无机盐、葡萄糖、半胱氨酸、维生素B_1和生长素(IAA)的培养基中,便增殖成一大块紊乱的组织,这些繁殖体叫做愈伤组织。每隔一定的时间分切一小块愈伤组织,放在新鲜培养基中,可以进行无限期的培养。差不多在同时 Chapter 3 Cultivation of Callus 3.1 History Gautheret and Nobecourt in the late 1930s In Europe, White pioneered a technology in the United States that revolutionized plant tissue culture. Gautheret reported in 1939 that the explants of carrot roots proliferated into large, disorganized tissues in media containing inorganic salts, glucose, cysteine, vitamin B_1, and auxin (IAA), which are called Callus. Cut a small piece of callus at regular intervals and place in fresh medium for indefinite growth. Almost at the same time