论文部分内容阅读
目的:探讨细胞外基质蛋白转化生长因子β诱导(TGFBI)对胃癌细胞SGC-7901转移潜能的影响。方法:牛血清白蛋白(BSA)、纤维粘连蛋白(FN)及细胞外基质蛋白TGFBI包被96孔板后,于其上培养SGC-7901细胞。MTT法检测其与TGFBI粘附能力及粘附后的增殖能力。同法包被Transwell小室滤膜下表面,检测其诱导SGC-7901细胞迁移的能力。结果:FN和TGFBI支持SGC-7901细胞粘附的能力明显高于BSA(P=0.023),FN和TGFBI之间差异无统计学意义,P>0.05;BSA、FN和TGFBI诱导SGC-7901细胞增殖的能力渐次增强,P=0.031;FN和TGFBI诱导SGC-7901细胞迁移的能力明显高于BSA(P=0.019),FN和TGFBI之间差异无统计学意义,P>0.05。结论:TGFBI可以增强胃癌细胞SGC-7901的转移潜能。
Objective: To investigate the effect of extracellular matrix protein transforming growth factor beta (TGFBI) on the metastatic potential of gastric cancer cell SGC-7901. Methods: BSA, fibronectin (FN) and extracellular matrix protein (TGFBI) were coated on 96-well plates and cultured on SGC-7901 cells. MTT assay with TGFBI adhesion capacity and proliferation after adhesion. The same method was used to coat the lower surface of Transwell chamber filter to test its ability to induce SGC-7901 cell migration. Results: The ability of FN and TGFBI to support SGC-7901 cell adhesion was significantly higher than that of BSA (P = 0.023), but there was no significant difference between FN and TGFBI (P> 0.05). The proliferation of SGC-7901 cells was induced by BSA, FN and TGFBI (P = 0.031). There was no significant difference between FN and TGFBI (P> 0.05). The ability of FN and TGFBI to induce SGC-7901 cell migration was significantly higher than that of BSA (P = 0.019). Conclusion: TGFBI can enhance the metastatic potential of gastric cancer cell SGC-7901.