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[目的]探讨丙烯腈亚慢性染毒对雄性大鼠精浆果糖和锌含量的影响以及果糖、锌含量与精液运动参数的相关关系。[方法]SPF级健康成年雄性SD大鼠40只,按体重随机分为对照组(玉米油)、低剂量组(丙烯腈12.5 mg/kg)、中剂量组(丙烯腈25 mg/kg)和高剂量组(丙烯腈50 mg/kg),每组10只。灌胃染毒,1次/d,6 d/周,连续13周。于末次染毒后采用颈椎脱臼法分批处死大鼠,检测大鼠精浆中果糖、锌含量以及附睾精子密度、精子活率、精子活力。[结果]中、高剂量染毒组大鼠精囊腺和前列腺系数与对照组比较降低(P=0.045、0.011,P=0.048、0.004)。低、中、高剂量染毒组大鼠精浆果糖、锌含量与对照组比较降低(P=0.002、0.000、0.000;P=0.013、0.016、0.001)。低、中、高剂量染毒组大鼠精子活率与对照组比较降低(P=0.043、0.004、0.014);高剂量染毒组大鼠精子密度、精子活力与对照组比较降低(P=0.007,P=0.031)。精浆果糖、锌含量与精子活率呈正相关(分别r=0.672,P=0.001;r=0.611,P=0.000)。[结论]在本实验条件下,丙烯腈亚慢性染毒可以导致雄性大鼠生殖内分泌系统损害,抑制雄性大鼠精囊腺、前列腺分泌果糖和锌,影响精子密度、活率和活力,从而对精子运动及受精能力产生不良影响。
[Objective] To investigate the effects of sub-chronic exposure to acrylonitrile on the content of fructose and zinc in seminal plasma of male rats and the relationship between the contents of fructose and zinc and semen movement parameters. [Method] Forty SPF healthy male SD rats were randomly divided into control group (corn oil), low dose group (acrylonitrile 12.5 mg / kg), middle dose group (acrylonitrile 25 mg / kg) High-dose group (acrylonitrile 50 mg / kg), 10 in each group. Gavage poisoning, 1 / d, 6 d / week for 13 weeks. After the last exposure, the rats were sacrificed in batches by cervical dislocation. The fructose, zinc content, epididymal sperm density, sperm motility and sperm motility were determined in seminal plasma of rats. [Results] The seminal vesicle and prostate coefficient of rats in medium and high dose groups were lower than those in control group (P = 0.045,0.011, P = 0.048,0.004). The contents of fructose and zinc in the seminal plasma of rats in low, medium and high dose groups were lower than those in the control group (P = 0.002,0.000,0.000; P = 0.013,0.016,0.001). The sperm motility of rats in low, medium and high dose groups was lower than that in control group (P = 0.043,0.004, 0.014). The sperm density and sperm motility in high dose group were lower than those in control group (P = 0.007 , P = 0.031). Serum fructose, zinc content and sperm motility was positively correlated (r = 0.672, P = 0.001; r = 0.611, P = 0.000). [Conclusion] Under this experimental condition, subchronic exposure to acrylonitrile could cause damage to reproductive and endocrine system of male rats, inhibit seminal vesicle and prostate secretion of fructose and zinc in male rats, affect sperm density, viability and vitality, Exercise and fertility adversely affected.